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CD8(+) T cells can block herpes simplex virus type 1 (HSV-1) reactivation from latency in sensory neurons.

TitleCD8(+) T cells can block herpes simplex virus type 1 (HSV-1) reactivation from latency in sensory neurons.
Publication TypeJournal Article
Year of Publication2000
AuthorsLiu T, Khanna KM, Chen X, Fink DJ, Hendricks RL
JournalJ Exp Med
Volume191
Issue9
Pagination1459-66
Date Published2000 May 1
ISSN0022-1007
KeywordsAnimals, CD8-Positive T-Lymphocytes, Cells, Cultured, Eye Infections, Viral, Female, Herpes Simplex, Herpesvirus 1, Human, Mice, Mice, Inbred BALB C, Neurons, Afferent, Trigeminal Ganglion, Virus Activation, Virus Latency
Abstract

<p>Recurrent herpes simplex virus type 1 (HSV-1) disease usually results from reactivation of latent virus in sensory neurons and transmission to peripheral sites. Therefore, defining the mechanisms that maintain HSV-1 in a latent state in sensory neurons may provide new approaches to reducing susceptibility to recurrent herpetic disease. After primary HSV-1 corneal infection, CD8(+) T cells infiltrate the trigeminal ganglia (TGs) of mice, and are retained in latently infected ganglia. Here we demonstrate that CD8(+) T cells that are present in the TGs at the time of excision can maintain HSV-1 in a latent state in sensory neurons in ex vivo TG cultures. Latently infected neurons expressed viral genome and some expressed HSV-1 immediate early and early proteins, but did not produce HSV-1 late proteins or infectious virions. Addition of anti-CD8alpha monoclonal antibody 5 d after culture initiation induced HSV-1 reactivation, as demonstrated by production of viral late proteins and infectious virions. Thus, CD8(+) T cells can prevent HSV-1 reactivation without destroying the infected neurons. We propose that when the intrinsic capacity of neurons to inhibit HSV-1 reactivation from latency is compromised, production of HSV-1 immediate early and early proteins might activate CD8(+) T cells aborting virion production.</p>

Alternate JournalJ. Exp. Med.
Citation KeyCK103
PubMed ID10790421
PubMed Central IDPMC2213436
Grant ListR01 EY005945 / EY / NEI NIH HHS / United States
EY05945 / EY / NEI NIH HHS / United States
EY11528 / EY / NEI NIH HHS / United States
P30 EY008098 / EY / NEI NIH HHS / United States
5P30EY08098 / EY / NEI NIH HHS / United States